Neural cell adhesion molecule 1 (NCAM1, also known as CD56) is a cell adhesion glycoprotein of the immunoglobulin (Ig) superfamily.
It is a multifunction protein involved in synaptic plasticity, neurodevelopment, and neurogenesis.
NCAM1 is expressed on human neurones, glial cells, skeletal muscle cells, NK cells and a subset of T cells, and the expression is observed in a wide variety human tumors, including myeloma, myeloid leukemia, neuroendocrine tumors, Wilms' tumor, neuroblastoma, and NK/T cell lymphomas.
Three major isoforms of NCAM1, with molecular masses of 120, 140, and 180 kD, are generated by alternative splicing of mRNA (PMID: 9696812).
The glycosylphosphatidylinositol (GPI)-anchored NCAM120 and the transmembrane NCAM140 and NCAM180 consist of five Ig-like domains and two fibronection-type III repeats (FNIII).
All three forms can be posttranslationally modified by addition of polysialic acid (PSA) (PMID: 14976519).
Several other isofroms have also been described.
Applications:Suitable for use in Flow Cytometry and Western Blot.
Other applications not tested.
Recommended Dilutions:Flow Cytometry: 1:20-1:100Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human NCAM1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human NCAM1.
