84-2216-99　［取扱停止］MLST8 (Mammalian Lethal with SEC13 Protein 8, G-Protein beta Subunit-like, GBL, LST8, MGC111011, POP3, WAT1) 100ul　212015

特徴

• Cell growth is a fundamental biological process whereby cells accumulate mass and increase in size.
• The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1).
• mTOR is a large protein kinase with two different complexes.
• One complex contains mTOR, GbetaL, and raptor, which is a target of rapamycin.
• The other complex, insensitive to rapamycin, includes mTOR, GbetaL, and rictor (1).
• GbetaL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2).
• A reduction in GbetaL expression has been shown to decrease in vivo phosphorylation of S6K1 (2).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.