84-2216-87　［取扱停止］MLH1 (Human Mul L Homologue, Familial Nonpolyposis Colin Cancer Type 2, COCA2, Colon Cancer Type 2, COCA2, FCC2, HNPCC, HNPCC2, MGC5172, hMLH1) 100ul　212009

特徴

• Mismatch repair (MMR), a conserved process that involves correcting errors made during DNA synthesis, is crucial to the maintenance of genomic integrity.
• MLH1 is the human homologue of the E.
• coli MMR gene mutL.
• MMR requires recognition of a base mismatch or insertion/deletion loop by a MutS homolog followed by recruitment of a MutL heterodimeric complex consisting of MLH1 and PMS1 (MutL-alpha), PMS2 (MutL-beta) or MLH3 (MutL-gamma).
• Other factors required for MMR in eukaryotes are EXO1, PCNA, RFC, RPA, DNA polymerases and DNA ligase (reviewed in 1).
• Inactivation of the MLH1 gene causes genome instability and predisposition to cancer (2-5).
• The MLH1 gene is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC) (6).
• MLH1 also plays a role in meiotic recombination.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.