84-2216-35　［取扱停止］MET, phosphorylated (Y1349) (Hepatocyte Growth Factor Receptor, HGF, AUTS9, Scatter Factor Receptor, HGFR, RCCP2, c-MET) 50ul　211981

特徴

• Met, a high affinity tyrosine kinase receptor for hepatocyte growth factor (HGF, also known as scatter factor) is a disulfide-linked heterodimer made of 45kD alpha- and 145kD beta-subunits (1,2).
• The alpha-subunit and the amino-terminal region of the beta-subunit form the extracellular domain.
• The remainder of the beta-chain spans the plasma membrane and contains a cytoplasmic region with tyrosine kinase activity.
• Interaction of Met with HGF results in autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl, and PI3 kinase (3).
• These fundamental events are important for all of the biological functions involving Met kinase activity.
• The addition of a phosphate at cytoplasmic Tyr1003 is essential for Met protein ubiquitination and degradation (4).
• Phosphorylation at Tyr1234/1235 in the Met kinase domain is critical for kinase activation.
• Phosphorylation at Tyr1349 in the Met cytoplasmic domain provides a direct binding site for Gab1 (5).
• Research studies have shown that altered Met levels and/or tyrosine kinase activities are found in several types of tumors, including renal, colon, and breast.
• Thus, investigators have concluded that Met is an attractive potential cancer therapeutic and diagnostic target (6,7).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.