Midkine, or MK, is a heparin-binding molecule involved in the regulation of growth and differentiation during embryogenesis.
MK expression is tightly regulated during embryonic development by steroid receptors of the retinoic acid superfamily.
The mature human MK protein is 118 amino acids in length and contains five intrachain disulfide bonds.
MK is a non-glycosylated protein that shows greater than 87% identity between human and mouse.
The carboxy-terminus of MK contains the principle heparin-binding site and the molecule’s neurite-promoting sequences; both the amino- and carboxy-terminal sequences are required for the molecule’s neurotrophic properties.
An association between overexpression of MK and colon adenocarcinoma has been shown in families suffering from familial polyposis.
In addition, MK functions to enhance the activity of plasminogen activator (PA).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry (Paraffin): 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol
Specificity：Recognizes human MDK. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human MDK.
