84-2213-76　［取扱停止］MAPK14 (Mitogen-activated Protein Kinase 14, MAP Kinase 14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A, EXIP, Mxi2, PRKM14, PRKM15, RK, p38, p38ALPHA) 100ul　211875

特徴

• p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4).
• Four isoforms of p38 MAPK, p38a, beta, gamma (also known as Erk6 or SAPK3), and delta (also known as SAPK4) have been identified.
• Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5).
• MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182.
• Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8).SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK.
• This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9).
• SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10).
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunocytochemistry: 1:50-1:100Immunoprecipitation: 1:10-1:100Flow Cytometry: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.