Lymphotoxin-alpha (LT-a), also known as TNF-beta, is a member of the TNF superfamily of proteins (1).
NK cells, T cells, B cells, and lymphoid tissue-inducer cells express LT-a (1).
LT-a can be secreted as a soluble homotrimer or form membrane bound heterotrimers with lymphotoxin-beta (LT a1b2 or LT a2b1) which can be cleaved from the cell surface by matrix metalloproteases (1,2).
Soluble LT-a binds to and signals through TNFR1/TNFR2, activating the canonical NF-kB pathway (1).
In contrast, LT a1b2 heterodimers bind to the LTbR receptor and activate the noncanonical NF-kB pathway (1).
As a result, LT-a and TNF-a have overlapping functions.
Soluble LT-a and LT a1b2 play key roles in lymphangiogenesis (3).
The LT a1b2/LTbR axis is essential for the development of lymphoid tissue (1,3).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human LTA.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human LTA.
