Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells.
Keratin expression is highly regulated, tissue specific, and varies according to cell-state.
Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40kD (KRT19) to 64kD (KRT9).
Type 2 keratins consist of basic or neutral, high molecular weight proteins with MW from 52kD (KRT8) to 67kD (KRT18).
KRT18, also named as CYK18, PIG46 and K18, is the most commonly found members of the intermediate filament gene family.
KRT18 is a type I keratin which is found primarily in non squamous epithelia and is present in a majority of adenocarcinomas and ductal carcinomas but not in squamous cell carcinomas.
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:100Immunoprecipitation: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KRT18.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human KRT18.
