KDM3A, also name as JMJD1A, JHDM2A, JMJD1, JMJD1A, KIAA0742 and TSGA, belongs to the JHDM2 histone demethylase family.
It is a histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code.
It is involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin.
It is involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.
(PubMed:16603237), JMJD1A is a useful biomarker for hypoxic tumor cells and a prognostic marker that could be a promising therapeutic target against CRC.
(PMID:20823141) This antibody recognizes the N-terminal of KDM3A (JMJD1A).
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry, ChIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:100Immunoprecipitation: 1:50-1:100Immunoprecipitation: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human KDM3A.
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human KDM3A.
