84-2206-28　［取扱停止］Interferon, gamma (IFNG, IFG, IFI, IFN-y) 50ul　211521

特徴

• Interferons (IFNs) appear both locally and systematically early after viral infection and participate in limiting the spread of infection.
• They also affect cell differentiation, growth, surface antigen expression and immunoregulation (1).
• There are three naturally occurring interferons: alpha, beta and gamma.
• IFN-alpha is derived from lymphoblastic tissue and has a number of therapeutic applications in the treatment of various human cancers and diseases of viral origin.
• Recombinant IFN-a from both natural and synthetic genes binds to a common cell surface receptor and induces antiviral activity in a variety of cell lines.
• When binding to discrete cell surface receptors on target cells, IFN-a induces rapid changes in Jak/Stat phosphorylation, which initiates the Jak/Stat signaling pathway (2).
• IFN-a signaling also involves production of DAG without an increased intracellular free calcium concentration and the subsequent activation of calcium-independent isoforms of PKC (beta and epsilon) (3).
• All IFN-a signaling pathways lead to final alterations of gene expression, which mediate their pleiotropic biologic activities
• IFNG, also known as type II interferon, is produced mainly in activated T lymphocytes and natural killer cells (4) and has broad effects on various cells of the immune system.
• Many signaling proteins including IL-2, FGF, and EGF induce the synthesis of IFNG.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.