84-2206-08　［取扱停止］Integrin alpha (ITGA4, CD49D, IA4, MGC90518) 100ul　211511

特徴

• Integrins are alpha/beta heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2).
• The integrin family contains at least 18 alpha and 8 beta subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3).
• Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).A pair of important a4 integrins, a4b1 and a4b7, interact with VCAM-1, fibronectin, and MAdCAM-1 at cell adhesions (3).
• Gene knockout and antibody blocking research reveal that a4 integrins play important roles in embryonic liver and heart development and in fetal lymphocyte homing (4-6).
• Phosphorylation at Ser988 within the cytoplasmic tail of integrin a4 blocks binding to paxillin and promotes leading edge migration (7,8).On SDS-PAGE, integrin a4 can migrate at several different apparent molecular sizes, a 150kD mature protein and a 140kD precursor protein (a 180kD protein also exists under mild non-reducing conditions) (9).
• Integrin a4 has a cleavage site at Arg558, which results in a small portion of the protein as either an 80kD N-terminal or 70kD C-terminal fragment (10).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.