Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730).
IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80kD IL-6-binding subunit (IL6R, CD126, gp80) and a 130kD signal-transducing element (gp130, CD130).
After binding of IL-6 to membrane-bound IL6R, the complex of IL-6 and IL6R associates with gp130, thus activating the receptor (PMID: 9716487).
Expression of gp130 is found in almost all organs while expression of IL6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).
Soluble form of IL6R has been found in human serum and urine.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IL6R.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human IL6R.
