The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation.
PA700, PA28, and PA200 are three major protein Interleukin-1beta (IL-1beta), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1).
It is produced primarily by activated monocytes and macrophages.
It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6).
Human IL-1beta is synthesized as a 31kD precursor.
To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17kD mature form (7,8).
Detection of the 17kD mature form of IL-1beta is a good indicator of caspase-1 activity.
Applications:Suitable for use in Western Blot and Immunofluorescence.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human IL1B.
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu
Immunogen：Recombinant fusion protein containing a sequence corresponding to amino acids 1-269 of human IL1B.
