84-2204-89　［取扱停止］IL1A (Interleukin 1 alpha, IL-1A, IL1, IL1-Alpha, IL1F1) 100ul　211463

特徴

• IL-1alpha is a pro-inflammatory cytokine produced by activated monocytes, lymphocytes and epithelial cells (1).
• IL-1a is synthesized as an active precursor protein that appears to be cleaved by cytosolic proteases into its mature form (1,2).
• Often, precursor and mature forms of IL-1a are primarily retained intracellularly rather than constitutively secreted.
• (1,2).
• Signaling by IL-1a involves IL-1a binding to an IL-1 accessory protein (IL-1-AcP) and then the complex binds to IL-1RI (1,2).
• Signaling is through activation of MAP kinase and NFkB pathways (1,2).
• IL-1a also binds to an IL-RII that lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor.
• Inhibition of IL-1a activity is through IL-1R antagonist (IL-1Ra) that binds IL-1R1 but does not signal.
• IL-1a has been shown to be a key mediator of virus-induced inflammatory responses in mice (3).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.