IDH1 is one of three isocitrate dehydrogenases that catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (alpha-KG).
These enzymes exist in two distinct subclasses that utilize either NAD or NADP+ respectively, as an electron acceptor (1).
IDH1 is the NADP+-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes.
IDH2 and 3 are mitochodrial enzymes that also function in the Krebs cycle.
IDH1 is inactivated by phosphorylation at Ser113 and contains a clasp-like domain wherein both polypeptide chains in the dimer interlock (2,3).
IDH1 is expressed in a wide range of species and also in organisms that lack a complete citric acid cycle.
Recently, an inactivating mutation of IDH1 has been implicated in glioblastoma (4).
IDH1 appears to function as a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway (5).
Applications:Suitable for use in Immunofluorescence,Western Blot, Immunoprecipitation, Immunohistochemistry, ChIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Immunoprecipitation: 1:50-1:200Immunoprecipitation: 1:20-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes IDH1. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human IDH1.
