84-2201-72　［取扱停止］HNRNPU (Heterogeneous Nuclear Ribonucleoprotein U, HNRPU, SAF-A, U21.1, hnRNP U) 200ul　211330

特徴

• This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
• The hnRNPs are RNA binding proteins and they form complexes with heterogeneous nuclear RNA (hnRNA).
• These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
• While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm.
• The hnRNP proteins have distinct nucleic acid binding properties.
• The protein encoded by this gene contains a RNA binding domain and scaffold-associated region (SAR)-specific bipartite DNA-binding domain.
• This protein is also thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes.
• During apoptosis, this protein is cleaved in a caspase-dependent way.
• Cleavage occurs at the SALD site, resulting in a loss of DNA-binding activity and a concomitant detachment of this protein from nuclear structural sites.
• But this cleavage does not affect the function of the encoded protein in RNA metabolism.
• At least two alternatively spliced transcript variants have been identified for this gene.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.