84-2198-85　［取扱停止］HES1 (Transcription Factor HES1, FLJ20408, HES-1, HHL, HRY, bHLHb39) 100ul　211216

特徴

• The Drosophila Hairy and enhancer of split genes encode basic helix-loop-helix (bHLH) transcriptional repressors that function in the Notch signaling pathway and control segmentation and neural development during embryogenesis.
• The mammalian homolog of Drosophila Hairy and enhancer of split are the HES gene family members HES1-6, which also encode bHLH transcriptional repressors that regulate myogenesis and neurogenesis.
• The HES family members form a complex with TLE, the mammalian homolog of groucho, and this interaction is mediated by the carboxy-terminal WRPW motif of the HES proteins.
• The HES/TLE complex functions by directly binding to DNA instead of interfering with activator proteins.
• Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG).
• HES2 binds to both N and E box sites, while HES6 does not bind DNA.
• Rather, HES6 inhibits HES1 activity, thereby promoting transcription.
• HES1 and HES2 are expressed in a variety of adult and embryonic tissues.
• HES3 is expressed exclusively in cerebellar Purkinje cells and HES5 is found solely in the nervous system.
• HES6 is produced in brain as well as in the limb buds of developing embryos.
• Applications:Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry, Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Flow Cytometry: 1:20-1:50Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.