Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA.
The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2).
HAT complexes interact with sequence-specific activator proteins to target specific genes.
In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3).
In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4).
Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5).
Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2.
Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7)
HDAC3 is a nuclear and cytoplasmic protein that deacetylates both histone (H2A, H3, H4) and non-histone substrates (RelA, SRY, p53, MEF2, PCAF and p300/CBP) (8).
HDAC3 deacetylase activity is stimulated by interactions with the N-CoR and SMRT co-repressor proteins.
Together, these three proteins form a functional complex that represses transcription associated with nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD and Pit-1 (8,9).
Phosphorylation of HDAC3 on Ser424 by casein kinase 2 (CK2) also increases HDAC3 deacetylase activity (9).
Subsequently, de-phosphorylation by protein phosphatase 4 (PP4) decreases HDAC3 activity (9).
Applications:Suitable for use in Immunofluorescence,Western Blot, Immunoprecipitation, Immunohistochemistry, ChIP.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:50-1:200Immunoprecipitation: 1:50-1:200Immunoprecipitation: 1:20-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes HDAC3. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：ChIP IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human HDAC3.
