Macrophage inhibitory cytokine-1 (Mic-1), also termed GDF15 (1), PTGF-beta (2), PLAB (3), PDF (4), and NAG-1 (5), is a divergent member of the transforming growth factor-beta (TGF-beta) superfamily (6).
Like other family members, Mic-1 is synthesized as an inactive precursor that undergoes proteolytic processing involving removal of an N-terminal hydrophobic signal sequence followed by cleavage at a conserved RXXR site generating an active C-terminal domain that is secreted as a dimeric protein.
Mic-1 is highly expressed in the placenta and is also dramatically increased by cellular stress, acute injury, inflammation, and cancer.
In the brain, Mic-1 is found in the choroid plexus and is secreted into the cerebrospinal fluid (7).
It is also a transcriptional target of the p53 tumor suppressor protein and may serve as a biomarker for p53 activity (8,9).
During tumor progression, Mic-1 has various effects on apoptosis, differentiation, angiogenisis, and metastasis, and may also contribute to weight loss during cancer (10,11).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human GDF15.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human GDF15 .
