Fgr is a member of the Src tyrosine kinase family.
It has a membrane-associated amino-terminal domain that is highly divergent from other family members, internal conserved SH2 and SH3 domains and a highly conserved carboxy-terminal tyrosine kinase catalytic domain (1,2).
Tyrosine 412 is located in the activation loop, and phosphorylation of this residue is critical for the activation of Fgr tyrosine kinase activity.
c-Fgr is predominantly expressed in cells of hematopoietic origin including differentiated myeloid cells, NK and B cells (3,4).
Fgr plays an important role in the signaling cascade from membrane receptors lacking intrinsic tyrosine kinase activity such as Bcr, FcR, and the integrin family of receptors (5).
It was demonstrated that Fgr functions as a selective inhibitor of beta2 integrin-mediated signaling and Syk kinase function in monocytes (5).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human FGR.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human FGR.
