84-2189-76　［取扱停止］FAS, phosphorylated (Y291) (TNF Receptor Superfamily Member 6, APT1, CD95, FAS1, APO-1, FASTM, ALPS1A, TNFRSF6) 200ul　210809

特徴

• Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2).
• Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4).
• FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40kD upon glycosylation) that belongs to the TNF family, which also includes TNF-alpha, TRAIL, and TWEAK.
• Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5).
• Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis.
• Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6).
• FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7).
• Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26kD trimeric soluble form (8,9).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.