Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells.
EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1).
One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue.
Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Synthetic peptide corresponding to human EpCAM (Ep-CAM).
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human EpCAM.
