Dynamin-related protein 1 (DNM1L) is a member of the dynamin superfamily of GTPases.
Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1).
DNM1L affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5).
The yeast ortholog of DNM1L clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3).
The division of the mitochondria, which is required for apoptosis, as well as normal cell growth and development is controlled, in part, by the phosphorylation of DNM1L at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6).
When phosphorylated at Ser616, DNM1L stimulates mitochondrial fission during mitosis.
Conversely, fission is inhibited when DNM1L is phosphorylated at Ser637 (reviewed in 6).
Dephosphorylation at Ser637 by calcineurin reverses this inhibition (7).
In addition to phosphorylation, sumoylation of DNM1L is also an enhancer of mitochondrial fission (8).
Balancing fission and fusion events is essential for proper mitochondrial function.
Research studies have demonstrated mitochondrial defects in a variety of neurodegenerative diseases including Alzheimer’s disease, Parkinson’s disease, and Huntington’s disease (reviewed in 6).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes DNM1L. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human DNM1L.
