Apoptosis is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units.
DNA fragmentation factor (DFF), heterodimer of 40kD (DFFB) and 45kD (DFFA) subunits, is one of the major endonucleases responsible for internucleosomal DNA cleavage during apoptosis.
DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis.
DFF activated after cleavage of DFFA by caspase-3 followed by its dissociation from DFFB.
DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.
DFFA appears to be a 45kD protein despite the predicated MW of 37kD.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes DFFA. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human DFFA.
