84-2177-69　［取扱停止］CRYGD (Gamma Crystallin D, CCP, PCC, CACA, CCA3, CRYG4, CTRCT4, Cry-g-D) 100ul　210269

特徴

• Crystallins are separated into two classes: taxon-specific, or enzyme, and ubiquitous.
• The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens.
• Since lens central fiber cells lose their nuclei during development, these crystallins are made and then retained throughout life, making them extremely stable proteins.
• Mammalian lens crystallins are divided into alpha, beta, and gamma families; beta and gamma crystallins are also considered as a superfamily.
• Alpha and beta families are further divided into acidic and basic groups.
• Seven protein regions exist in crystallins: four homologous motifs, a connecting peptide, and N- and C-terminal extensions.
• Gamma-crystallins are a homogeneous group of highly symmetrical, monomeric proteins typically lacking connecting peptides and terminal extensions.
• They are differentially regulated after early development.
• Four gamma-crystallin genes (gamma-A through gamma-D) and three pseudogenes (gamma-E, gamma-F, gamma-G) are tandemly organized in a genomic segment as a gene cluster.
• Whether due to aging or mutations in specific genes, gamma-crystallins have been involved in cataract formation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.