CREB is a bZIP transcription factor that activates target genes through cAMP response elements.
CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses.
While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system.
CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3).
Additionally, CREB signaling is involved in learning and memory in several organisms (4-6).
CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners.
CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling.
Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunocytochemistry: 1:50-1:100Immunoprecipitation: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CREB1. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human CREB1.
