p27 Kip1 is a member of the Cip/Kip family of cyclin-dependent kinase inhibitors.
Like its relatives, p57 Kip2 and p21 Waf1/Cip1, the ability to enforce the G1 restriction point is derived from its inhibitory binding to CDK2/cyclin E and other CDK/cyclin complexes.
Expression levels of p27 are upregulated in quiescent cells and in cells treated with cAMP or other negative cell cycle regulators.
Downregulation of p27 can be induced by treatment with interleukin-2 or other mitogens; this involves phosphorylation of p27 and its degradation by the ubiquitin-proteasome pathway (1-4)
p57 Kip2 (Cyclin-dependent kinase inhibitor 1C) functions as a tumor suppressor.
Mutations of p57 Kip2 have been associated with numerous human malignancies as well as Beckwith–Wiedemann syndrome (BWS), characterized by an increased risk of childhood cancer.
The amino-terminal CDK inhibitory domain, common to the family, binds to and inhibits CDK/cyclin complexes and restricts cell cycle progression (5).
The unique central region of p57 Kip2 interactes with LIMK-1, a downstream effector of the Rho family of GTPases.
By sequestering LIMK-1 in the nucleus, p57 Kip2 disrupts actin dynamics within cells and may be linked to its essential role in embryonic development (6).
In addition, the carboxyl-terminal QT domain of p57KIP2 binds to and inhibits JNK/SAPK activity regulating cellular apoptosis and differentiation (7).
Expression levels of human p57 Kip2 are more restricted then other CDK inhibitors (8) and are controlled by ubiquitination/degradation via the Skp1/Cul1/F-box-type E3 ubiquitin ligase complex SCF-Skp2.
This effect is dependent on Thr310 (9).
A similar threonine phosphorylation site in p27 Kip1, Thr187, has also been shown to regulate protein stability (10).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human CDKN1C.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human CDKN1C.
