The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression.
It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases.
In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1).
However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2).
The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3).
Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4).
Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).
Applications:Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunoprecipitation: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CDKN1A. Species Crossreactivity: human, rat
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to human CDKN1A.
