Cadherins are calcium-dependent cell adhesion proteins which preferentially interact with themselves in a homophilic manner in connecting cells, and thus may contribute to the sorting of heterogeneous cell type.
E-cadherin (E-Cad), also known as CDH1 and CD324, is the classical cadherin molecule and contains five cadherin domains of ~110aa each in the extracellular domain.
E-Cad is expressed in the non-neural epithelial tissues as disulfide-linked homodimer, and plays a key role in the organization and integrity of most epithelial tissues, cell differentiation and tissue development.
CDH1 interacts directly, via the cytoplasmic domain, with CTNNB1 or JUP to form the PSEN1/cadherin/catenin adhesion complex which connects to the actin skeleton through the actin binding of alpha-catenin.
During apoptosis or with calcium influx, E-Cad is cleaved by the metalloproteinase to produce fragments of about 38kD (E-CAD/CTF1), 33kD (E-CAD/CTF2) and 29kD (E-CAD/CTF3), respectively.
E-Cad has been identified as a potent invasive suppressor, as downregulation of E-cadherin expression is involved in dysfunction of the cell-cell adhesion system, and often correlates with strong invasive potential and poor prognosis of human carcinomas (gastric, breast, ovary, endometrium and thyroid).
Applications:Suitable for use in Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:200-1:500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes mouse CDH1.
Isotype：IgG
Calc Applications Abbrev：IHC
Calc Crossreactivity：Mo
Immunogen：Recombinant protein corresponding to mouse CDH1.
