Borealin is a 280-amino acid protein that stabilizes the bipolar mitotic spindle.
Borealin is part of a complex called Aurora B kinase that has essential regulatory roles at centromeres and the central spindle in cells undergoing mitosis.
This complex makes up the enzymatic core of the chromosomal passenger complex (CPC).
It is possible that the Borealin subunit directs the CPC to centromeric DNA.
Deletion of Borealin delays mitotic progression and causes kinetochore-spindle to mis-attach.
Deletion also causes an increase in bipolar spindles associated with ectopic asters.
These extra poles, which form after chromosomes acquire their bipolar orientation, seriously disrupt chromosome allotment during anaphase.
It is true that inappropriate chromosomal segregation and cell division may cause auneuploidy leading to cancer, so an abnormal expression of Borealin may lead to gastric cancer.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CDCA8. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human CDCA8 .
