84-2172-20　［取扱停止］CDC20 (Cell Division Cycle Protein 20 Homolog, CDC20A, MGC102824, bA276H19.3, p55CDC) 50ul　210022

特徴

• The cell division cycle demands accuracy to avoid the accumulation of genetic damage.
• This process is controlled by molecular circuits called "checkpoints" that are common to all eukaryotic cells (1).
• Checkpoints monitor DNA integrity and cell growth prior to replication and division at the G1/S and G2/M transitions, respectively.
• The cdc2-cyclin B kinase is pivotal in regulating the G2/M transition (2,3).
• Cdc2 is phosphorylated at Thr14 and Tyr15 during G2-phase by the kinases Wee1 and Myt1, rendering it inactive.
• The tumor suppressor protein retinoblastoma (Rb) controls progression through the late G1 restriction point (R) and is a major regulator of the G1/S transition (4).
• During early and mid G1-phase, Rb binds to and represses the transcription factor E2F (5).
• The phosphorylation of Rb late in G1-phase by CDKs induces Rb to dissociate from E2F, permitting the transcription of S-phase-promoting genes.
• In vitro, Rb can be phosphorylated at multiple sites by cdc2, cdk2, and cdk4/6 (6-8).
• DNA damage triggers both the G2/M and the G1/S checkpoints.
• DNA damage activates the DNA-PK/ATM/ATR kinases, which phosphorylate Chk at Ser345 (9), Chk2 at Thr68 (10) and p53 (11).
• The Chk kinases inactivate cdc25 via phosphorylation at Ser216, blocking the activation of cdc2.CDC20 binds to and activates the anaphase-promoting complex (APC) during mitosis and G1 phase of the cell cycle (12).
• Moreover, CDC20 is necessary for ubiquitin ligase activity of the APC/cyclosome (APC/C).
• In metaphase MAD2L1 inactivates the CDC20-APC/C complex, while in anaphase this inhibition is lost and CDC20-APC/C degrades its substrates (13).
• p53 and p21 suppress expression of CDC20 upon genotoxic stresses and ectopic introduction of p53.
• siRNA mediated knock-down of CDC20 in cancer cells leads to attenuated cell growth and induces G(2)/M arrest, suggesting that CDC20 is a possible therapeutic target of cancer (14).
• Organization of neuronal circuits requires presynaptic axonal differentiation and synapse formation.
• CDC20-APC regulates presynaptic differentiation in postmitotic neurons by triggering the required degradation of the transcription factor NeuroD2 (15)
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.