84-2170-70　［取扱停止］CCNE1, phosphorylated (T395) (Cyclin E1, CCNE) 200ul　209951

特徴

• The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle.
• Cyclins function as regulators of CDK kinases.
• Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event.
• This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition.
• This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase.
• Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.
• This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical role in promoting cell-cycle progression in the absence of pRB.
• Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have been described.
• Two additional splice variants were reported but detailed nucleotide sequence information is not yet available.
• Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunohistochemistry: 1:50-1:200Immunofluorescence: 1:20-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.