Caspase 12 is an enzyme known as a cysteine protease.
It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues.
It is most highly related to members of the ICE subfamily of caspases that process inflammatory cytokines.
Caspase-12 is located in the endoplasmic reticulum (ER).
It is responsible for ER-stress-induced apoptosis, such as high calcium concentration, low oxygen and low glucose levels.
Caspase-12 Antibody detects endogenous levels of full-length caspase-12 protein (50kD) and its cleaved product (40-44kD).
This antibody does not cross-react with other caspases.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:1000Immunohistochemistry: 1:20-1:200Immunofluorescence: 1:10-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CASP12. Species Crossreactivity: human, mouse
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human CASP12.
