84-2169-41　［取扱停止］CASP1 (Caspase-1, ICE, IL1BC, P45) 50ul　209887

特徴

• Caspase-1, or interleukin-1b converting enzyme (ICE/ICEa), is a class I cysteine protease, which also includes caspases -4, -5, -11, and -12.
• Caspase-1 cleaves inflammatory cytokines such as pro-IL-1beta and interferon-gamma inducing factor (IL-18) into their mature forms (1,2).
• Like other caspases, caspase-1 is proteolytically activated from a proenzyme to produce a tetramer of its two active subunits, p20 and p10.
• Caspase-1 has a large amino-terminal pro-domain that contains a caspase recruitment domain (CARD).
• Overexpression of caspase-1 can induce apoptosis (3).
• Mice deficient in caspase-1, however, have no overt defects in apoptosis but do have defects in the maturation of pro-IL-1beta and are resistant to endotoxic shock (4,5).
• At least six caspase-1 isoforms have been identified, including caspase-1 a, b, g, d, e and z (6).
• Most caspase-1 isoforms (a, b, g and d) produce products between 30-48kD and induce apoptosis upon over-expression.
• Caspase-1 epsilon typically contains only the p10 subunit, does not induce apoptosis and may act as a dominant negative.
• The widely expressed zeta isoform of caspase-1 induces apoptosis and lacks 39 amino-terminal residues found in the alpha isoform (6).
• Activation of caspase-1 occurs through an oligomerization molecular platform designated the "inflammasome" that includes caspase-5, Pycard/Asc, and NALP1 (7).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.