Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes.
A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits.
CAPN5 is one of the large subunits.
Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV.
Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.Tested applications: ELISA, WBName of antibody: CAPN5Host: Rabbit polyclonal antibodyReactivity: Human, Mouse, RatImmunogen: Recombinant protein corresponding to a region derived from 344-496 amino acids of Human Calpain-5Content(mg/ml): 0.8Full name: Calpain-5Synonyms: HTRA3, nCL-3 Genbank No.: BC018123ELISA Recommended dilution: 1000-5000WB Predicted band size: 73WB positive control: MCF-7 cellWB Recommended dulition: 200-1000Gel concentration: 8WB Lysates（ug）: 30WB Primary antibody actual dilution: 400WB Exposure time: 30 seconds
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CAPN5. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human CAPN5.
