Homologous to the poliovirus receptor (PVR/CD155), the Nectin immunoglobulin superfamily comprises four known isoforms, Nectin 1, 2, 3 and 4 (also designated TSLC1).
TSLC1 is encoded by a tumor-suppressor gene in human non-small-cell lung cancer mapping to chromosome 11q23.2.
The TSLC1 protein is an N-linked membrane glycoprotein that colocalizes with the Actin filament-binding protein, afadin, at cadherin-based adherens junctions in MDCKII epithelial cells.
TSLC1 also interacts with the tumor-suppressor gene product DAL-1 (for differentially expressed in adenocarcinoma of the lung protein 1) to target Actin rearragement and cellular motility.
TSLC1 may also form homodimers that function in homophilic, intracellular adhesion.
TSLC1 expression is reduced or absent in a number of characterized cancer cell lines including A549.
In prostate and breast cancer, as well as in pancreatic ductal adenocarcinoma, the TSLC1 promoter is commonly silenced by hypermethylation.
Unlike other Nectins, which are more widely expressed, TSLC1 is mainly expressed in the placenta.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes CADM1. Species Crossreactivity: human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human CADM1.
