84-2166-18　［取扱停止］BCL2L11 (Bcl-2-like Protein 11, Bcl2-L-11, Bcl2-interacting Mediator of Cell Death, BAM, BIM, BOD, BimL, BimS, BimEL, BIM-beta6, BIM-beta7, BIM-alpha6) 100ul　209741

特徴

• BCL2L11/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains (1,2).
• BCL2L11 induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family.
• Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1 and BHRF-1 (1,2).
• BCL2L11 functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which BCL2L11 expression is elevated (3-6).
• Three major isoforms of BCL2L11 are generated by alternative splicing: BCL2L11EL, BCL2L11L and BCL2L11S (1).
• The shortest form, BCL2L11S, is the most cytotoxic and is generally only transiently expressed during apoptosis.
• The BCL2L11EL and BCL2L11L isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis (7).
• Apoptotic activity of these longer isoforms may be regulated by phosphorylation (8,9).
• Environmental stress triggers BCL2L11 phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.