BCL2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release.
It has been implicated in modulating mitochondrial calcium homeostasis and proton flux.
Several phosphorylation sites have been identified within BCL2 including Thr56, Ser70, Thr74 and Ser87.
It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of BCL2 may be a marker for mitotic events.
Mutation of BCL2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes.
Interleukin 3 and JNK-induced BCL2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions.
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500-1:2000Immunohistochemistry (Paraffin): 1:50-1:200Immunofluorescence: 1:50-1:200Immunoprecipitation: 1:20-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BCL2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to human BCL2.
