84-2165-91　［取扱停止］BCL10 (B Cell Leukemia 10, CARMEN, CIPER, CLAP, c-E10, mE10) 200ul　209731

特徴

• Bcl10/CIPER/CLAP/mE10 is a widely expressed CARD (caspase recruitment domain) containing protein shown to induce apoptosis and activate NF-kB (1-5).
• The CARD domain mediates self-oligomerization, interactions with other CARD proteins and is necessary for NF-kB activation, although the precise mechanism which Bcl10 regulates these processes is not fully understood.
• The discovery of Bcl10 came from observations of the chromosomal translocation t(1;14)(p22;q32) from B cell lymphomas of the mucosa-associated lymphoid tissue (MALT) (1,5).
• This translocation results in deregulated expression of a truncated form of Bcl10 which lacks apoptotic activity and enhances transformation.
• Studies from Bcl10 deficient mice demonstrate that Bcl10 is essential for the activation of NF-kB by T- and B-cell receptors (6).
• One third of Bcl10 deficient mice developed lethal exencephaly.
• Surviving mice were unaffected by various apoptotic stimuli, but were severely immunodeficient and defective in antigen receptor-induced NF-kB activiation.
• PKC or T-cell receptor signaling results in a downregulation of Bcl10 protein levels, attenuating both NF-kB activation and cellular proliferation and also provides a negative feedback regulation of the NF-kB signaling to T cell signaling (7).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.