Bak is a proapoptotic member of the Bcl-2 family (1).
This protein is located on the outer membrane of mitochondria and is an essential component for transduction of apoptotic signals through the mitochondrial pathway (2,3).
Upon apoptotic stimulation, an upstream stimulator like truncated BID (tBID) induces conformational changes in Bak to form oligomer channels in the mitochondrial membrane for cytochrome c release.
The release of cytochrome c to the cytosol activates the caspase-9 pathway and eventually leads to cell death (4,5).
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence/Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence(IC): 1:50-1:200Immunoprecipitation: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human BAK1. Species Crossreactivity: mouse, rat
Isotype：IgG
Calc Applications Abbrev：IC IF IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to a sequence within aa62-112 from human BAK1.
