84-2164-25　［取扱停止］ATP1A1 (ATPase alpha Subunit Isoform 1, MGC3285, MGC51750, Na, K-ATPase) 200ul　209654

特徴

• The Na,K-ATPase is an integral membrane heterodimer belonging to the P-type ATPase family.
• This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving sodium export and potassium import across the plasma membrane against their electrochemical gradients.
• It is composed of a catalytic alpha subunit and a beta subunit (reviewed in 1).
• Several phosphorylation sites have been identified for the a1 subunit.
• Tyr10 is phosphorylated by an as yet undetermined kinase (2), Ser16 and Ser23 are phosphorylated by PKC, and Ser943 is phosphorylated by PKA (3-5).
• All of these sites have been implicated in the regulation of enzyme activity in response to hormones and neurotransmitters, altering trafficking and kinetic properties of Na,K-ATPase.
• Altered phosphorylation in response to angiotensin II stimulates activity in the rat proximal tubule (6).
• Na,K-ATPase is also involved in other signal transduction pathways.
• Insulin regulates its localization in differentiated primary human skeletal muscle cells, and this regulation is dependent on ERK1/2 phosphorylation of the alpha subunit (7).
• Na,K-ATPase and Src form a signaling receptor complex that affects regulation of Src kinase activity and, subsequently, its downstream effectors (8,9).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.