Cool/Pix proteins comprise a family of guanine nucleotide exchange factors (GEFs) localized to focal adhesions.
The family consists of two isoforms, cool2/alpha pix and cool1/betaPix, the latter having two splice variants that vary in their carboxy termini (1).
Cool1/betaPix, like other GEFs, has a DH (Dbl homology) domain, which allows binding of small GTPases and GDP/GTP exchange, and a PH (Pleckstrin homology) domain, which is important in regulating subcellular localization.
Cool1/betaPix also has an SH3 domain, which binds to the PAK kinase, a downstream effector of cdc42 and Rac (3,4).
Phosphorylation of cool1/betaPix by PAK2 downstream of MAPK signaling alters the localization of a complex containing PAK2 and cool-1/betaPix, regulating formation of growth cones in response to growth factors (4).
Growth factor induced activation of Rac1 via cool1/betaPix was later shown to occur independently of subcellular localization (5).
Endothelin-1 stimulation of mesangial cells stimulates the protein kinase A (PKA) pathway, resulting in translocation of cool-1/betaPix and activation of cdc42 (6).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes ARHGEF7. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human ARHGEF7.
