84-2161-63　［取扱停止］APEX1 (Apurinic-apyrimidinic Endonuclease 1, AP Endonuclease 1, APEX nuclease, APE, APE1, APEN, APEX, APX, HAP1, REF1) 100ul　209539

特徴

• Ape1 (Apurinic/Apyrimidic eEndonuclease 1), also known as Ref1 (Redox effector factor 1), is a multifunctional protein with several biological activities.
• These include roles in DNA repair and in the cellular response to oxidative stress.
• Ape1 initiates the repair of abasic sites and is essential for the base excision repair (BER) pathway (1).
• Repair activities of Ape1 are stimulated by interaction with XRCC1 (2), another essential protein in BER.
• Ape1 functions as a redox factor that maintains transcription factors in an active, reduced state but can also function in a redox-independent manner as a transcriptional cofactor to control different cellular fates such as apoptosis, proliferation and differentiation (3).
• Increased expression of Ape1 is associated with many types of cancers including cervical, ovarian, prostate, rhabdomyosarcomas and germ cell tumors (4).
• Ape1 has been shown to stimulate DNA binding of several transcription factors known to be involved in tumor progression such as Fos, Jun, NF-kB, PAX, HIF-1, HLF and p53 (4).
• Mutation of the Ape1 gene has also been associated with amyotrophic lateral sclerosis (ALS) (5,6).
• Applications:Suitable for use in Immunofluorescence and Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months after receipt.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.