Annexin V, also known as PAP-1 or Lipocortin V, is a ~30kD protein that binds to phospholipids in a calcium-dependent manner (1).
All annexins contain a putative PKC binding site, but only annexin V has been identified as an inhibitor of this pathway (2).
It may also signal, by direct interaction with VEGFR2 receptor, in the regulation of vascular endothelial cell proliferation (3).
Annexin V preferentially binds phosphatidylserine, in competition with prothrombin, leading to inhibition of blood coagulation at sites of injury preceding contact between lipid components and coagulation factors that initiate thrombosis (4-6).
The ability of Annexin V to bind specifically and robustly to hosphatidylserine makes it an attractive reagent in detecting apoptotic cells (7).
Annexin V is inducible by glucocorticoids and can be phosphorylated by tyrosine and serine/threonine kinases (8).
It is thought to block production of mediators of inflammation, such as prostaglandins and leukotrienes by inhibiting the release of arachidonic acid from membranes by phospholipase A2 (8).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes ANXA5. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human ANXA5.
