The aldehyde dehydrogenase family is a large group of enzymes that oxidize aldehydes formed through metabolic processes to their carboxylic acids (1).
ALDH1A1 is a liver cytosolic isoform of acetaldehyde dehydrogenase and is involved in the major pathway of alcohol metabolism along with alcohol dehydrogenase (2).
ALDH1A1 is also known as retinal dehydrogenase 1 and is involved in retinol metabolism, converting retinol to retinoic acid (3).
Recent studies suggest that control of retinoid signaling through ALDH1A1 may influence hematopoietic stem cell differentiation (4).
There has been recent interest in ALDH1 isoforms as predictive biomarkers in disease.
Several studies have suggested that ALDH1A1 is a potential marker for cancer stem cells and chemoresistance in several tumor types, such as melanoma (5), lung cancer (6), glioblastoma (7).
Applications:Suitable for use in Immunofluorescence and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Immunofluorescence: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes human ALDH1A1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IF WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant fusion protein corresponding to amino acids 48-147 of human ALDH1A1.
