alpha-Actinin belongs to the spectrin family of cytoskeletal proteins.
It was first recognized as an actin cross-linking protein, forming an antiparallel homodimer with an actin binding head at the amino terminus of each monomer.
More recently, alpha-actinin has been shown to interact with a large number of proteins involved in signaling to the cytoskeleton including those involved in cellular adhesion, migration, and immune cell targeting (1).
The interaction of alpha-actinin with intercellular adhesion molecule-5 (ICAM-5) helps to promote neurite outgrowth (2).
In osteoblasts, interaction of alpha-actinin with integrins stabilizes focal adhesions and may protect cells from apoptosis (3).
Isoforms 1 and 4 of alpha-actinin, which are non-muscle isoforms, are present in stress fibers, sites of adhesion and intercellular contacts, filopodia, and lamellipodia.
The muscle isoforms 2 and 3 localize to the Z-discs of striated muscle and to dense bodies and plaques in smooth muscle (1).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500-1:2000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes ACTN1. Species Crossreactivity: human, mouse, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human ACTN1.
