5-Methylcytosine is the methylated form of the DNA base cytosine.
In mammalian cells, DNA methylation is an enzymatic modification at the 5-position of cytosine present abundantly within the CpG dinucleotide sequence context.
This DNA modification is inheritable and reversible without primary DNA base sequence changes, resulting in possible epigenetic modulation of phenotype and gene expression (1).
CpG methylation may directly interfere with the binding of certain transcriptional regulators to their cognate DNA sequences or may enable recruitment of methyl-CpG binding proteins that create a repressed chromatin environment (2).
The de novo formation and maintenance of 5-methylcytosine is catalyzed by DNA methyltransferase proteins (DNMTs) (1).
Applications:Suitable for use in MeDIP, Immunocytochemistry Other applications not tested.
Recommended Dilution:MeDIP1:1000-1:2000Immunocytochemistry: 1:50-1:200Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Specificity：Recognizes 5-Methylcytosine. Species Crossreactivity: human, mouse, rat
