Human Bone Morphogenetic Protein 2 (BMP2) is a disulfide-bonded homodimeric protein with an apparent molecular weight of 26kD.
BMP2 regulates similiarly to its nearest homologue BMP-4 diverse fundamental processes during embryonic development: BMP2 and other BMP proteins have great potential for medical therapeutic applications, in particular because they allow or at least accelerate the ossification of extensive bone lesions.
BMP2 lacks the natural N-terminus which results in a 15-20 fold increase of specific activity.
BMP2 is a heparin binding protein.
Applications:Suitable for use in ELISA, Western Blot and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA: 0.5-2ug/ml (100ul/well); Detects 0.2-0.4ng/well of recombinant hBMP-2
Western Blot: 0.1-0.2ug/ml; Detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
Neutralization: 1.3-1.9ug/ml; Yields one-half maximal inhibition [ND50] of the biological activity of hBMP-2 (200ng/ml)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human Bone Morphogenetic Protein 2 (BMP-2).
Isotype：IgG
Calc Applications Abbrev：E N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human BMP-2, expressed in E. coli.
