84-2156-01　VEGF (Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGFA, VPF) (HRP) 100ul　V2110-09A-HRP

特徴

• Applications:Suitable for use in Neutralization, ELISA, Immunohistochemistry and Western Blot.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry (Formalin-fixed paraffin embedded): 5-15ug/ml with a 30 minute incubation at RT or an overnight incubation at 4ºC
• Neutralization: 0.05-0.1ug/ml yields one-half maximal inhibition [ND50] of the biological activity (10ng/ml)
• ELISA (Indirect amd Sandwich): 0.5ug/ml (100ul/well).
• Used in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well of recombinant hVEGF
• Western Blot: 0.1-0.2ug/ml.
• Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.