TNF RII (p75, CD120b) is a 75kD transmembrane glycoprotein originally isolated from a human lung fibroblast library.
Among the multitude of cells known to express TNFRII are monocytes, endothelial cells, Langerhans cells, and macrophages.
Mouse to human amino acid sequence identity in the TNFRII cytoplasmic domain is 73%, while aa sequence identity in the extracellular region falls to 58%.
This drop in extracellular identity is reflected in the observation that human TNF-alpha is not active in the mouse system.
TNF RII to TNF RI, amino acid sequence identity is only about 20% in the extracellular region and 5% in the cytoplasmic domain.
TNF RII consists of a 240aa residue extracellular region, a 27aa residue transmembrane segment and a 173aa residue cytoplasmic domain.
TNF R1 and TNF R2 are members of a family of structurally related membrane receptors that includes lymphotoxin receptor, Fas, WSL1, DR4, CD40, CD30, CD27, 4-1BB, OX40, and p75 nerve growth factor receptor.
Members of the TNFR family can interact through their cytoplasmic domains with a range of intracellular signalling proteins, most of which fall into two distinct groups.
The first is the death domain containing proteins, including TRADD, FADD/MORT1, and RIP, which associate directly with receptors also containing death domains, such as TNF R1 and Fas.
The second is the TRAF proteins.
TRAF1 and TRAF2 were originally identified by their association with the cytoplasmic domain of TNFR2.
TRAF proteins appear to function as adaptor proteins.
TRAF2 directly binds at least eight intracellular molecules, including TRAF1, cIAP1, cIAP2, I-TRAF/TANK, A20, TRIP, RIP, and NIK.
The best characterized TRAFmediated signal transduction pathway is the activation of NF-B transcription factors.
TRAF2 mediates NF-B activation via the recruitment of the serine/threonine kinase NIK, which can in turn activate CHUK, an IBspecific kinase that triggers IB degradation.
In addition to recruiting mediators of NF-B activation, TRAF2 can bind at least three other molecules (I-TRAF/TANK, A20, TRIP) that inhibit its ability to activate NF-B.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Neutralization.
Other applications not tested.
Recommended Dilution:ELISA: To detect hsTNF-receptor II by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5-2ug/ml of this antibody is required.
This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor II as a detection antibody, allows the detection of at least 0.2-0.4ng/well of recombinant hsTNF-receptor II
Western Blot: To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2ug/ml.
Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3ng/lane, under either reducing or non-reducing conditions
Immunohistochemistry: This antibody stained PC-3 and CaCo-2 cells.; The primary antibody was incubated at 3mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody
Neutralization: To yield one-half maximal inhibition of the biological activity of Human sTNF Receptor-II (0.5ug/ml) a concentration of 15-20ug/ml of this antibody is required
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human sTNF Receptor Type II.
Isotype：IgG
Calc Applications Abbrev：E IHC N WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human sTNF Receptor Type II, expressed in E. coli.
